This article describes the development and validation of a new reverse phase HPLC method for the estimation of vinblastine in pharmaceutical formulations. Vinblastine, a Vinka alkaloid and a chemical analogue of vincristine, is generally used in combination therapy with synthetic molecules. The new method uses a mobile phase consisting of 90% methanol, 5% acetonitrile, and 5% of 0.1% orthophosphoric acid, with a pH maintained at 3.5. Analysis is carried out using PEAK 7000 isocratic HPLC with rheodyne manual sample injector with a switch. The resultant chromatogram obtained has a high resolution and low tailing factor of 1.29. The linearity curve showed a correlation coefficient (r2) of 0.999 for a wide range of drug concentrations of 3-15 ppm. The method was also validated in respect of precision, accuracy, and specificity. The applicability of the method was also tested with a commercial sample of Cytoblastin. Overall, this method is more accurate, precise, and convenient in terms of linearity, accuracy, precision, and others.
Author(s) Details:
P. Rama Krishna Veni,
Department of Applied Sciences and Humanities, Sasi Institute of Technology & Engineering, Tadepalligudem – 534101, Andhra Pradesh, India.
N. Sharmila,
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India.
B. Hari Babu,
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur – 522510, Andhra Pradesh, India.
Please see the link here: https://stm.bookpi.org/ACPR-V4/article/view/13007
Keywords: Vinblastine, estimation, RP-HPLC, cytoblastin