For bacterial analyses, one ml sample is taken, diluted in a 9 ml sterile seawater solution (10−1) and grind with a mortar. Then 1ml of the diluted sample solution is taken and placed in a 9 ml sterile tube and shaken until homogeneous to obtain a dilution of 10−2, in the same way, dilutions of 10−3,10−4,10−5,10−6 are carried out. Next, 100𝜇m sample of 10−3 was taken into a Petri dish filled with Zobel media and spread evenly prior to being wrapped in sterile paper and incubated for 2×24 hours. Pure bacteria cultures obtained from the streak method were used to identify bacteria strain by observing morphological features of the colony followed by biochemical tests (oxidative test, catalase, Hugh-Leison test, H2 S, indol, methyl red, Vogen-Proskauer test, nitrate reduction, citrate, sugar, decarboxylase and carbohydrate hydrolysis) according to Krieg et al.,. The method of English et al. was used for coral juvenile identification; Luning, Lima et al. and Muslihah et al. for macroalgae.
Author(s) Details:
Norma Afiati
Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang, Semarang, Indonesia.
Pujiono W. Purnomo
Faculty of Fisheries and Marine Science, Diponegoro University, Tembalang, Semarang, Indonesia.
Recent Global Research Developments in Coral Community Analysis in Lembeh Island
“Assessing Coral Reef Resilience on Lembeh Island, North Sulawesi” by Idris et al. (2022) explores the resilience of coral reef ecosystems on Lembeh Island. The study considers parameters such as bleaching resistance, taxonomic diversity, and fish abundance. Lembeh Island shows high potential for recovery from natural stressors [1].
“Rapid Transition in the Structure of a Coral Reef Community: The Effects of Coral Bleaching and Physical Disturbance” discusses the impacts of coral bleaching and physical disturbances on coral reef communities. This study sheds light on the changing structure of reef ecosystems [2].
“Change in Coral Reef Benthic Communities in the Lembeh Strait and Likupang, North Sulawesi, Indonesia” examines the composition of coral reef benthic communities, including shifts from coral-dominated to algal-dominated states. Anthropogenic impacts and natural disturbances play a significant role in these changes [3].
References
- Adiwijaya, C. “Assessing of coral reef resilience on Lembeh Island, North Sulawesi.” In IOP Conference Series: Earth and Environmental Science, vol. 967, no. 1, p. 012054. IOP Publishing, 2022.
- Hadi, T.A., Sihouka, J., Shi, X. et al. Change in coral reef benthic communities in the Lembeh Strait and Likupang, North Sulawesi, Indonesia. Acta Oceanol. Sin. 37, 45–54 (2018). https://doi.org/10.1007/s13131-018-1287-0
- Hadi, T. A., Sihouka, J., Shi, X., Budiyanto, A., & Suharsono. (2018). Change in coral reef benthic communities in the Lembeh Strait and Likupang, North Sulawesi, Indonesia. Acta Oceanologica Sinica, 37, 45-54.
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